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foxp3 1x perm solution  (Thermo Fisher)


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    Structured Review

    Thermo Fisher foxp3 1x perm solution
    ( A-B ) Overview of experimental design. 3’ scRNA-seq <t>(10X</t> Chromium v2 and v3) was performed on dissociated tibialis anterior (TA) muscles from young (4-7 mo), old (20 mo), and geriatric (26 mo) mice (both sexes) 0-7 days post-notexin injury (dpi) with n = 3-4 replicates per age and dpi ( B ). ( C ) Processing workflow. Each scRNA-seq sample was aligned to the mm10 mouse reference genome, ambient RNA was removed by SoupX, low quality cells were identified and removed, and doublets were identified and removed. All samples were then integrated with Harmony, resulting in a final dataset containing 273,923 cells from 65 samples. See Supplementary Figure 1 and Extended Data File 1 for additional detail. ( D ) Fraction of cells from each age group. ( E ) Fraction of cells from each dpi within each age group. ( F - G ) UMAP representations of the final dataset. Cells colored by manually assigned cell type IDs based on the expression of hallmark skeletal muscle genes (see Supplementary and ) ( F ). Cells are colored by age group, with all other cells in gray ( G ).
    Foxp3 1x Perm Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/foxp3 1x perm solution/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    foxp3 1x perm solution - by Bioz Stars, 2026-03
    86/100 stars

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    1) Product Images from "Single-cell transcriptomic analysis of skeletal muscle regeneration across mouse lifespan identifies altered stem cell states associated with senescence"

    Article Title: Single-cell transcriptomic analysis of skeletal muscle regeneration across mouse lifespan identifies altered stem cell states associated with senescence

    Journal: bioRxiv

    doi: 10.1101/2023.05.25.542370

    ( A-B ) Overview of experimental design. 3’ scRNA-seq (10X Chromium v2 and v3) was performed on dissociated tibialis anterior (TA) muscles from young (4-7 mo), old (20 mo), and geriatric (26 mo) mice (both sexes) 0-7 days post-notexin injury (dpi) with n = 3-4 replicates per age and dpi ( B ). ( C ) Processing workflow. Each scRNA-seq sample was aligned to the mm10 mouse reference genome, ambient RNA was removed by SoupX, low quality cells were identified and removed, and doublets were identified and removed. All samples were then integrated with Harmony, resulting in a final dataset containing 273,923 cells from 65 samples. See Supplementary Figure 1 and Extended Data File 1 for additional detail. ( D ) Fraction of cells from each age group. ( E ) Fraction of cells from each dpi within each age group. ( F - G ) UMAP representations of the final dataset. Cells colored by manually assigned cell type IDs based on the expression of hallmark skeletal muscle genes (see Supplementary and ) ( F ). Cells are colored by age group, with all other cells in gray ( G ).
    Figure Legend Snippet: ( A-B ) Overview of experimental design. 3’ scRNA-seq (10X Chromium v2 and v3) was performed on dissociated tibialis anterior (TA) muscles from young (4-7 mo), old (20 mo), and geriatric (26 mo) mice (both sexes) 0-7 days post-notexin injury (dpi) with n = 3-4 replicates per age and dpi ( B ). ( C ) Processing workflow. Each scRNA-seq sample was aligned to the mm10 mouse reference genome, ambient RNA was removed by SoupX, low quality cells were identified and removed, and doublets were identified and removed. All samples were then integrated with Harmony, resulting in a final dataset containing 273,923 cells from 65 samples. See Supplementary Figure 1 and Extended Data File 1 for additional detail. ( D ) Fraction of cells from each age group. ( E ) Fraction of cells from each dpi within each age group. ( F - G ) UMAP representations of the final dataset. Cells colored by manually assigned cell type IDs based on the expression of hallmark skeletal muscle genes (see Supplementary and ) ( F ). Cells are colored by age group, with all other cells in gray ( G ).

    Techniques Used: Expressing



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    foxp3 1x perm solution  (Thermo Fisher)
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    Thermo Fisher foxp3 1x perm solution
    ( A-B ) Overview of experimental design. 3’ scRNA-seq <t>(10X</t> Chromium v2 and v3) was performed on dissociated tibialis anterior (TA) muscles from young (4-7 mo), old (20 mo), and geriatric (26 mo) mice (both sexes) 0-7 days post-notexin injury (dpi) with n = 3-4 replicates per age and dpi ( B ). ( C ) Processing workflow. Each scRNA-seq sample was aligned to the mm10 mouse reference genome, ambient RNA was removed by SoupX, low quality cells were identified and removed, and doublets were identified and removed. All samples were then integrated with Harmony, resulting in a final dataset containing 273,923 cells from 65 samples. See Supplementary Figure 1 and Extended Data File 1 for additional detail. ( D ) Fraction of cells from each age group. ( E ) Fraction of cells from each dpi within each age group. ( F - G ) UMAP representations of the final dataset. Cells colored by manually assigned cell type IDs based on the expression of hallmark skeletal muscle genes (see Supplementary and ) ( F ). Cells are colored by age group, with all other cells in gray ( G ).
    Foxp3 1x Perm Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/foxp3 1x perm solution/product/Thermo Fisher
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    1x fix/perm solution foxp3 staining kit  (Thermo Fisher)
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    ( A-B ) Overview of experimental design. 3’ scRNA-seq <t>(10X</t> Chromium v2 and v3) was performed on dissociated tibialis anterior (TA) muscles from young (4-7 mo), old (20 mo), and geriatric (26 mo) mice (both sexes) 0-7 days post-notexin injury (dpi) with n = 3-4 replicates per age and dpi ( B ). ( C ) Processing workflow. Each scRNA-seq sample was aligned to the mm10 mouse reference genome, ambient RNA was removed by SoupX, low quality cells were identified and removed, and doublets were identified and removed. All samples were then integrated with Harmony, resulting in a final dataset containing 273,923 cells from 65 samples. See Supplementary Figure 1 and Extended Data File 1 for additional detail. ( D ) Fraction of cells from each age group. ( E ) Fraction of cells from each dpi within each age group. ( F - G ) UMAP representations of the final dataset. Cells colored by manually assigned cell type IDs based on the expression of hallmark skeletal muscle genes (see Supplementary and ) ( F ). Cells are colored by age group, with all other cells in gray ( G ).
    1x Fix/Perm Solution Foxp3 Staining Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x fix/perm solution foxp3 staining kit/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
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    Image Search Results


    ( A-B ) Overview of experimental design. 3’ scRNA-seq (10X Chromium v2 and v3) was performed on dissociated tibialis anterior (TA) muscles from young (4-7 mo), old (20 mo), and geriatric (26 mo) mice (both sexes) 0-7 days post-notexin injury (dpi) with n = 3-4 replicates per age and dpi ( B ). ( C ) Processing workflow. Each scRNA-seq sample was aligned to the mm10 mouse reference genome, ambient RNA was removed by SoupX, low quality cells were identified and removed, and doublets were identified and removed. All samples were then integrated with Harmony, resulting in a final dataset containing 273,923 cells from 65 samples. See Supplementary Figure 1 and Extended Data File 1 for additional detail. ( D ) Fraction of cells from each age group. ( E ) Fraction of cells from each dpi within each age group. ( F - G ) UMAP representations of the final dataset. Cells colored by manually assigned cell type IDs based on the expression of hallmark skeletal muscle genes (see Supplementary and ) ( F ). Cells are colored by age group, with all other cells in gray ( G ).

    Journal: bioRxiv

    Article Title: Single-cell transcriptomic analysis of skeletal muscle regeneration across mouse lifespan identifies altered stem cell states associated with senescence

    doi: 10.1101/2023.05.25.542370

    Figure Lengend Snippet: ( A-B ) Overview of experimental design. 3’ scRNA-seq (10X Chromium v2 and v3) was performed on dissociated tibialis anterior (TA) muscles from young (4-7 mo), old (20 mo), and geriatric (26 mo) mice (both sexes) 0-7 days post-notexin injury (dpi) with n = 3-4 replicates per age and dpi ( B ). ( C ) Processing workflow. Each scRNA-seq sample was aligned to the mm10 mouse reference genome, ambient RNA was removed by SoupX, low quality cells were identified and removed, and doublets were identified and removed. All samples were then integrated with Harmony, resulting in a final dataset containing 273,923 cells from 65 samples. See Supplementary Figure 1 and Extended Data File 1 for additional detail. ( D ) Fraction of cells from each age group. ( E ) Fraction of cells from each dpi within each age group. ( F - G ) UMAP representations of the final dataset. Cells colored by manually assigned cell type IDs based on the expression of hallmark skeletal muscle genes (see Supplementary and ) ( F ). Cells are colored by age group, with all other cells in gray ( G ).

    Article Snippet: After incubating we followed the manufacturer’s protocol (FOXP3 Transcription factor fixation/permeabilization kit, eBioscience # 00-5521-00) and cells were washed with staining buffer, spun, and resuspended in FoxP3 1x perm solution (10x Permeabilization buffer, Invitrogen # 00-8333-56) and incubated for 30 minutes at 4C in the dark.

    Techniques: Expressing